Biotechnology: Principle and ProcessMedium

Question

The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ' $X$ ' and ' $Y$ ' regions:

Options

A.The gene ' $X$ ' is for protein involved in replication of Plasmid and ' $Y$ ' for resistance to antibiotics.
B.Gene ' $X$ ' is responsible for recognitions sites and ' $Y$ ' is responsible for antibiotic resistance.
C.The gene ' $X$ ' is responsible for resistance to antibiotics and ' $Y$ ' for protein involved in the replication of Plasmid.
D.The gene ' $X$ ' is responsible for controlling the copy number of the linked DNA and ' $Y$ ' for protein involved in the replication of Plasmid.

Solution

Sol. [Key Concept: ori and rop in pBR322]

X marks ori, the origin of replication, which is the cis acting DNA sequence where replication initiates and which influences linked DNA copy number. Y marks rop, a protein coding gene involved in regulation of plasmid replication. Hence, option (4). Strictly, ori is a DNA site rather than a protein coding gene.

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