Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of a

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Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of antibiotic resistance towards:

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Sol. [Key Concept: pBR322 — insertional inactivation at the BamHI (tetR) site] If one ligate a foreign DNA at the BamHI site of tetracycline resistance gene in the vector pBR322, the recombinant plasmid will lose tetracycline resistance due to insertion of foreign DNA.

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