Biotechnology: Principle and ProcessMedium

Question

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R.

Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells.

Reason R: Isolation of genetic material needs disruption of cells.

In the light of the above statements, choose the most appropriate answer from the options given below:

Options

A.$\mathbf{A}$ is correct but $\mathbf{R}$ is not correct
B.$\mathbf{A}$ is not correct but $\mathbf{R}$ is correct
C.Both $\mathbf{A}$ and $\mathbf{R}$ are correct and $\mathbf{R}$ is the correct explanation of $\mathbf{A}$
D.Both A and R are correct but R is not the correct explanation of A

Solution

Sol. [Key Concept: Assertion–Reason evaluation]

Since, the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This can be achieved by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).

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