Question
Which of the following statements are correct with respect to DNA separation, isolation and visualization?
A. The cutting of DNA is done by molecular scissors.
B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis.
C. The separated DNA fragments can be seen without staining when exposed to UV light.
D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light.
Choose the correct answer from the options given below:
Options
Solution
Sol. [Key Concept: Gel electrophoresis — separation of DNA fragments]
• The cutting of DNA is possible by the use of restriction enzymes that results in the fragments of DNA. These fragments of DNA can be separated by a technique known as gel electrophoresis.
- The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel.
- The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.
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