Question
Which of the following statements are not true regarding restriction endonucleases?
A. They are called molecular scissors.
B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli.
C. They cut the DNA only at the center of the palindromic sites.
D. They remove nucleotides only from the ends of DNA fragments.
E. They recognize specific palindromic base-pair sequences.
Choose the answer from the options given below:
Options
Solution
Sol. [Key Concept: Properties of restriction endonucleases]
Statements C and D are incorrect. Restriction endonucleases usually cut the DNA slightly away from the centre of palindrome sites. They cannot remove nucleotides from the ends of the DNA fragment, which is function of restriction exonuclease.
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