Cell: The Unit of LifeMedium

Question

Arrange the following steps of somatic hybridization in a correct sequence.

A. Digestion of cell walls.

B. Isolation of naked protoplasts.

C. Fusion of protoplasts to get hybrid protoplast.

D. Isolation of single cells from two different varieties of plants.

E. Growing of hybrid protoplast to form a new plant.

Choose the correct answer from the options given below:

Options

A.D, A, B, C, E
B.E, B, A, D, C
C.D, B, A, E, C
D.E, A, B, C, D

Solution

Sol. [Key Concept: Sequence of steps in somatic hybridisation]

Scientist have even isolated single cell protoplasts from two different varieties of plants each having a desirable character and after digesting their cell walls have been able to isolate naked protoplasts (surrounded by plasma membrane) that can be fused to get hybrid protoplast, which can be further grown to form a new plant. These hybrids are called somatic hybrids, while the process is called somatic hybridization.

So the correct sequence for the formation of somatic hybrids is D, A, B, C, E.

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