Molecular Basis of InheritanceMedium

Question

Arrange the following steps of DNA fingerprinting in a correct sequence.

A. Isolation of DNA and its digestion by restriction endonucleases.

B. Hybridisation using a labelled VNTR probe.

C. Transferring of separated DNA fragments to synthetic membranes.

D. Detection of hybridised DNA fragments by autoradiography.

E. Separation of DNA fragments by electrophoresis.

Choose the correct answer from the options given below :

Options

A.A, B, D, C, E
B.A, D, B, E, C
C.A, E, C, B, D
D.A, E, B, C, D

Solution

Sol. [Key Concept: Sequence of steps in DNA fingerprinting]

The following is the correct sequence of steps of DNA fingerprinting.

A. Isolation of DNA and its digestion by restriction endonuclease.

B. Probes made complementary to the VNTR locus are allowed to hybridize with the DNA fragments.

C. The separated DNA fragments are transferred to synthetic membranes made of nylon or nitrocellulose.

D. Finally, the hybridized DNA fragments are detected under X-rays in a technique called autoradiography.

E. DNA fragments are separated based on their size by the technique of gel electrophoresis.

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