Biotechnology: Principle and ProcessMedium
Question
In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
Options
A.Using ampicillin & tetracycline containing medium plate.
B.Blue color colonies will be selected.
C.White color colonies will be selected.
D.Blue color colonies grown on ampicillin plates can be selected.
Solution
The correct Ans. is that white-colored colonies will be selected.
Since an alien piece of DNA is being inserted at EcoRI site, the gene $\beta$-galactosidase present here will undergo insertional inactivation.
This gene is responsible for producing blue-colored colonies, but since it has been insertionally inactivated, white colored colonies will be produced.
Ampicillin and tetracycline resistance genes present in the given DNA will remain intact. Thus, the given DNA will show amp$\ ^{R}$ and tet$\ ^{R}$.
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