Microbes in Human WelfareMedium
Question
What is the first steep in the Sothern blot technique?
Options
A.Denaturation of DNA on the gel for hybridization with specific probe.
B.Production of a group of genetically identicalcells.
C.Digestion of DNA by restriction enzyme.
D.Denaturation of DNA from a nucleated cell such as the one from the scene of crime.
Solution
The Southern bolt is used to detest and identycertain DNA sequences in a sample of bodily fluid. It uses single-stranedDNA to search out their complementry strands. When Southern blot is performed on Dna, the first step is digestion of DNA with restriction enzymes. Restriction enzymes cut DNA at known sequences, and produces DNA fragments of a certain lenght. Once the DNA is cut into pieces, scientists contuct electrophoresis to separate them by size.
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