Microbes in Human WelfareHard
Question
Gel electrophoresis is used for
Options
A.cutting of DNA into fragments
B.separation of DNA fragments according to their size
C.construction of recombinant DNA by joining with cloning vectors
D.isolation of DNA molecule
Solution
When genomic DNA extracted from any tissue of a plant or animal species digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves movement of fragments or molecules from a well created on one edge of the gel.
Endonucleases are enzyme that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves Dna only within or mear those sites, which have specific case sequences, such endonucleases are known as restriction endonucleases.
The selected vector is cut poen with a restriction enzyme that has a unique target site located in the sequence wher the DNA insert should be integrated to facilitate the selection of recombinant DNA.
Endonucleases are enzyme that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves Dna only within or mear those sites, which have specific case sequences, such endonucleases are known as restriction endonucleases.
The selected vector is cut poen with a restriction enzyme that has a unique target site located in the sequence wher the DNA insert should be integrated to facilitate the selection of recombinant DNA.
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