Cry-I endotoxins obtained form Bacillus thuringiensis are effective against

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Insect resistant transgenic plants contain either a gene from the bacterium Bacillus thuringiensis or some other gene. The Cry gene of Bacillus thuringiensis produces a protein, which forms crystalline inclusion in the bacterial spores. These crystal proteins are responsible for tte insecti cidal activities of the bacterial strains. Cry-I endotoxins are effective against Lepidoplera (eg, flies). Cry-V and Cry-VI proteins are effective against nematodes. Cry-IV proteins are effec tive against Diptera (mosquitons ). When Cry proteins are ingested by insects, they are dis solved in the alkaline juices preesnt in the midgut lumen. The gut proteases process them hydrolytically to release the core toxic fragments. The toxic Fragments are believed to bind to specifi high affinity receotors present in the brush border of midgut epithelial cells and eventual lysis.

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