Cell Cycle and Cell DivisionMedium
Question
A student wishes to study thr cell structure under a light microscope having 10X eyepiece and 45X objective. He should illuminate the object by which one of the following colours of light so to get best possible resolution ?
Options
A.Blue
B.Gerrn
C.Yellow
D.Red
Solution
Resolving power or resolution is the ability of the lens to distinguish fine details and structure. Specifically it refers to the ability of the lanses to distinguish between two points a specified distance apart. Resolving powerv depends on two factrs :
(a) Wavelength of light used for illumnation.
(b) Power of objective lenses.
Since, the limit of resolving power of a microscope is fixed by the structure of light, the shortest wavelength of visible light will give the maximum resolution. Among yellow, green, red and blue libht colour. Blue (500nm) have shortest wavelength so, it will give best resolution.
(a) Wavelength of light used for illumnation.
(b) Power of objective lenses.
Since, the limit of resolving power of a microscope is fixed by the structure of light, the shortest wavelength of visible light will give the maximum resolution. Among yellow, green, red and blue libht colour. Blue (500nm) have shortest wavelength so, it will give best resolution.
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